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. 2021 Oct 1;8:748118. doi: 10.3389/fnut.2021.748118

Figure 5.

Figure 5

Effect of BTL-1 on MAPK signaling pathway. The impact of BTL-1 on the MAPK signaling pathway determined by qPCR (A–C). The outcome of BTL-1 on the MAPK signaling pathway determined by western blotting (D–G). Effect of P38 inhibitor (SB203580, 10 μM, 1 h) on the phosphorylation of P38. (H,I) determined by western blotting. Impact of P38 inhibitor on the mRNA and protein of TNF-α determined by qPCR and ELISA. (J,K). The outcome of ERK1/2 inhibitor (PD98095,50 μM, 1 h) on the phosphorylation of ERK1/2 (L,M) determined by western blotting analysis. Inhibitory effect of ERK1/2 inhibitor on the mRNA and protein of TNF-α determined by qPCR and ELISA (N,O). The data shown are representative of three independent experiments. The inhibitors of ERK1/2 and P38 were added 1 h before LPS and BTL-1 treatment, and the cells were washed with PBS three times before adding LPS and BTL-1. The results were expressed as the means ± SEM. #P < 0.05, ##P < 0.01, compared to non-treated group, *P < 0.05, **P < 0.01 compared to LPS group.