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. 2021 Oct 1;8:748118. doi: 10.3389/fnut.2021.748118

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Copyright © 2021 Chen, Zhang, Niu, Mohyuddin, Wen, Bao, Yu, Wu, Hu, Yong, Liu, Abd El-Aty and Ju.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro effect of BTL-1 on intestinal paracellular permeability. Transmembrane resistance of IPEC-J2 monolayer measured by IPEC-J2 cell resistance voltmeter (A). The effect of BTL-1 on transmembrane resistance in vitro intestinal paracellular permeability was measured by a fluorometer (B). The effect of BTL-1 on FITC-D fluorescence intensity in the lower compartment measured by a fluorescence microplate reader (C). The effect of BTL-1 on TJ protein determined by qPCR (D–F), IF (G), and western blotting (H–K). The data are representative of three independent experiments. The results were expressed as the means ± SEM. ^#P < 0.05, ^##P < 0.01, compared to non-treated group, ^*P < 0.05, ^**P < 0.01 compared to LPS group.