Table 1.
Primer sequence.
| Gene ID | Primer | Sequence (5'-3') |
|---|---|---|
| 414396 | pig β-actin Forward | GCTGTCCCTGTATGCCTCT |
| pig β-actin Reverse | GATGTCACGCACGATTTCC | |
| 399500 | pig IL-6 Forward | ATAAGGGAAATGTCGAGGCTGTGC |
| pig IL-6 Reverse | GGGTGGTGGCTTTGTCTGGATTC | |
| 397122 | pig IL-1β Forward | CAAGCCAGAGAAGCAAGGTGTCC |
| pig IL-1β Reverse | GCCGTCCTCAGCAGCAAGAAG | |
| 397106 | pig IL-10 Forward | AGCCAGCATTAAGTCTGAGAACAGC |
| pig IL-10 Reverse | GGTCAGCAACAAGTCGCCCATC | |
| 397086 | pig TNF-α Forward | AAAGGACACCATGAGCACGGAAAG |
| pig TNF-α Reverse | CGCCACGAGCAGGAATGAGAAG | |
| 399541 | pig TLR4 Forward | GCCATCGCTGCTAACATCATC |
| pig TLR4 Reverse | CTCATACTCAAAGATACACCATCGG | |
| 100135665 | pig P65 Forward | CTGAGGCTATAACTCGCTTGGTGAC |
| pig P65 Reverse | CATGTCCGCAATGGAGGAGAAGTC | |
| 100157586 | pig IκB Forward | GGCAGTGACATGAGTGGCAGATAC |
| pig IκB Reverse | CTTCCTTGAGGCTGTGCTTCTTCC | |
| 445013 | pig ERK1 Forward | ACGTCATTGGCATCCGAGACATTC |
| pig ERK1 Reverse | GAGGAAGTAGCAGATGTGGTCGTTG | |
| 100626611 | pig P38 Forward | CGCCTGTGAAGACCTCCTTGAAC |
| pig P38 Reverse | TTCCTGTCCTCCACCTTCCGAAG | |
| 396610 | pig JNK Forward | ACTACAGAGCACCTGAGGTCATCC |
| pig JNK Reverse | ATTTCTCCCATAATGCACCCCACAG | |
| 397236 | pig Occludin Forward | CAGTGGTAACTTGGAGGCGTCTTC |
| pig Occludin Reverse | CGTCGTGTAGTCTGTCTCGTAATGG | |
| 100736682 | pig ZO-1 Forward | CCAGGGAGAGAAGTGCCAGTAGG |
| pig ZO-1 Reverse | TTTGGTGGGTTTGGTGGGTTGAC | |
| 100625166 | pig Claudin1 Forward | AGAAGATGCGGATGGCTGTCATTG |
| pig Claudin1 Reverse | ACCATACCATGCTGTGGCAACTAAG | |
| 11461 | mouse β-actin Forward | GTGACGTTGACATCCGTAAAGA |
| mouse β-actin Reverse | GCCGGACTCATCGTACTCC | |
| 16193 | mouse IL-6 Forward | CTCCCAACAGACCTGTCTATAC |
| mouse IL-6 Reverse | CCATTGCACAACTCTTTTCTCA | |
| 16176 | mouse IL-1β Forward | GAAATGCCACCTTTTGACAGTG |
| mouse IL-1β Reverse | TGGATGCTCATCAGGACAG | |
| 16153 | mouse IL-10 Forward | TTCTTTCAAACAAAGGACCAGC |
| mouse IL-10 Reverse | GCAACCCAAGTAACCCTTAAAG | |
| 21926 | mouse TNF-α Forward | ATGTCTCAGCCTCTTCTCATTC |
| mouse TNF-α Reverse | GCTTGTCACTCGAATTTTGAGA | |
| 21898 | mouse TLR4 Forward | GCCATCATTATGAGTGCCAATT |
| mouse TLR4 Reverse | AGGGATAAGAACGCTGAGAATT | |
| 19697 | mouse P65 Forward | TGCGATTCCGCTATAAATGCG |
| mouse P65 Reverse | ACAAGTTCATGTGGATGAGGC | |
| 12675 | mouse IκB Forward | TTGGGTTATGCCAAAGATGTTG |
| mouse IκB Reverse | GCTGTGTACGGCTTATTTTCAA | |
| 26417 | mouse ERK1 Forward | CAGCTCAACCACATTCTAGGTA |
| mouse ERK1 Reverse | TCAAGAGCTTTGGAGTCAGATT | |
| 26416 | mouse P38 Forward | AGGAATTCAATGACGTGTACCT |
| mouse P38 Reverse | AGGTCCCTGTGAATTATGTCAG | |
| 26419 | mouse JNK1 Forward | TTGAAAACAGGCCTAAATACGC |
| mouse JNK1 Reverse | GTTTGTTATGCTCTGAGTCAGC | |
| 18260 | mouse Occludin Forward | TGCTTCATCGCTTCCTTAGTAA |
| mouse Occludin Reverse | GGGTTCACTCCCATTATGTACA | |
| 21872 | mouse ZO-1 Forward | CTGGTGAAGTCTCGGAAAAATG |
| mouse ZO-1 Reverse | CATCTCTTGCTGCCAAACTATC | |
| 12737 | mouse Claudin1 Forward | AGATACAGTGCAAAGTCTTCGA |
| mouse Claudin1 Reverse | CAGGATGCCAATTACCATCAAG |