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. 2021 Oct 1;12:766782. doi: 10.3389/fphar.2021.766782

FIGURE 3.

FIGURE 3

Suppressive effects of WS-3 on ED development and epileptic seizures in WT mice, and deterioration of ED development and epileptic seizures resulting from lack of TRPM8 channels. (A) Experimental protocol. Mice were anesthetized with sevoflurane. The power of the low beta band in ECoGs was integrated for 5 min during basal activity (25–30 min) and the pre- (55–60 min) and post- (145–150 min) injection periods. Examples of changes in EDs with (B) PG + 1% DMSO (n = 5), (C) PG + 1.0 mM WS-3 (n = 6) in WT mice, and (D) PG + 1% DMSO (n = 5), and (E) PG + 1.0 mM WS-3 (n = 5) in TRPM8KO mice. (B–E) Representative ECoG traces for 5 s during basal activity and the pre- and post-injection periods. (B–E) Typical behavior during seizure monitoring. (F) The integrated value of the low beta band in the contralateral side during basal activity (white) and the pre-injection (black) period. (G) Mean seizure scores that were at the maximum during the 60-min period for vehicle (white) or WS-3 (grey) administration in WT mice and for vehicle (black) or WS-3 (grey-oblique line) administration in TRPM8KO (black) mice. The results are shown as mean ± SEM; **p < 0.01, ***p < 0.001, followed by Tukey’s test; † p < 0.05, †† p < 0.01, Student’s t-test; ‡ p < 0.05, Welch’s t-test.