Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 1;9:727972. doi: 10.3389/fcell.2021.727972

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Shaheen, Akhtar, Umer, Khan, Bakhtiari, Saleem, Faisal and Tariq.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Mutants of TCP-1 ring complex (TRiC) exhibit Polycomb group (PcG)-like behavior. (A–D) Heterozygous male flies for Pc (+/Pc¹ and +/Pc^XL5) obtained by crossing Pc (Pc¹ and Pc^XL5) alleles with w¹¹¹⁸ exhibit strong extra sex comb phenotype, and these heterozygous males were used as control. CCT7 (CCT7^KG01477) and CCT5 (CCT5⁰⁶⁴⁴⁴) mutants crossed to two different alleles of Pc (Pc¹ and Pc^XL5) significantly enhanced extra sex comb phenotype in CCT7^KG01477/Pc (A,B) and CCT5⁰⁶⁴⁴⁴;Pc (C,D) mutants, respectively, when compared with control (+/Pc¹ and +/Pc^XL5). Here, 200 male flies with desired genotype from the progeny of each cross were scored for extra sex combs. Flies were categorized based on the number of extra sex comb bristles on the second and third pairs of legs (Tariq et al., 2009) as follows: –, no extra sex combs on second or third pair of legs; +, 1–2 bristles on the second leg; ++, 3 or more bristles on the second leg; +++, 3 or more bristles on the second leg and 1–2 bristles on the third leg; ++++, strong sex combs on both second and third pairs of legs. Percentages of flies for each category of phenotype, heterozygous Pc¹/+ and Pc^XL5/+ males and CCT7^KG01477/Pc¹ and CCT7^KG01477/Pc^XL5 double mutant male flies, are provided in Supplementary Table 1. The percentage of flies for each category was plotted as bar graphs. Error bars represent standard error of the mean (SEM) from three independent experiments. Statistical significance was calculated using t-test (* p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001). (E,F) CCT7/trx¹ double mutant males from progeny of CCT7 mutants crossed to trx¹ were scored for suppression of A5 to A4 transformation. As compared to wild-type flies (E, left panel), heterozygous trx mutant (+/trx¹) males obtained from the cross of w¹¹¹⁸ with trx¹ mutant exhibit loss of abdominal pigmentation on A5 segment (E, middle panel), i.e., phenotype referred to as A5 to A4 transformation, and appearance of bristle on sternite 6 (s6, marked with black arrow). As compared to trx heterozygous (+/trx¹) male flies, CCT7 mutations suppressed A5 to A4 transformation phenotypes in CCT7/trx¹ double mutant male (E, right panel) flies. Flies were categorized into wild-type (E, left panel) and A5 to A4 transformation (E, middle panel) based on abdominal pigmentation phenotype. Percentage of flies obtained for each category of phenotype was plotted as bar graphs (F). Error bars represent SEM from two independent experiments. Statistical significance was calculated using two-way ANOVA (*** p ≤ 0.001).