FIGURE 3.

BRG1 deficiency down-regulates TBK1 phosphorylation via c-SRC. (A) Primary hepatocytes isolated from WT and BRG1^LKO mice were transduced with indicated adenovirus followed by treatment with PA (0.4 mM). TBK1 phosphorylation was examined by Western. (B,C) WT and BRG1^LKO mice were fed an HFHC diet or a chow diet for 16 weeks. SRC expression in the liver was examined by qPCR and Western. Each lane represents a sample extracted from an individual mouse. *p < 0.05 (one-way ANOVA with post hoc Scheffe test for qPCR quantification and two-tailed student’s t-test for Western quantification). (D,E) WT and BRG1^LKO mice were fed an MCD diet or a chow diet for 8 weeks. SRC expression in the liver was examined by qPCR and Western. Each lane represents a sample extracted from an individual mouse. *p < 0.05 (one-way ANOVA with post hoc Scheffe test for qPCR quantification and two-tailed student’s t-test for Western quantification). (F,G) Primary hepatocytes were isolated from WT and BRG1^LKO mice and treated with palmitate (PA, 0.4 mM) for 12 h. SRC expression was examined by qPCR and Western. *p < 0.05 (one-way ANOVA with post hoc Scheffe test). Data are expressed as mean ± standard deviation (SD). All experiments were performed in triplicate wells and repeated three times. One representative experiment is shown.