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. 2021 Sep 29;12:100085. doi: 10.1016/j.mbplus.2021.100085

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© 2021 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — Phenylephrine-induced phosphorylation of MYPT1 was enhanced in Col8^−/−VSMCs, and smooth muscle cell marker expression was increased. A-B, serum starved Col8^+/+ and Col8^−/− VSMCs were stimulated with 10 µM PE for 10 min and immunoblotted for pMYPTI/MYPT1. C, Col8^+/+ and Col8^−/− VSMCs were immunoblotted for VMSC markers. Quantification for αSMA (D); SM22 (E); Calponin (F); SM-MHC (G) is depicted. All band intensities were normalized to β-actin. Values represent the mean ± SEM (n = 3), *p < 0.05; **p < 0.01; ***p < 0.001.