Fig. 4.

Differential matrix composition in Col8^+/+ and Col8^−/−VSMCs in vitro. A-B, Expression levels for ECM genes related to elastic fiber proteins (Eln, Lox, Fbn1, Fbn2, Efemp2, Fbln5, Emilin1, Magp1), collagens (Col1a1, Col1a2, Col3a1, Col5a1, Col5a2, Col5a3), and fibronectin were determined by qRT-PCR. C, Decellularized matrices were labeled for either elastin, fibronectin or collagen-I. Scale bar = 100 µM. Mean intensity quantified for D, elastin (green); E, collagen-I (green); F, fibronectin (green). Values represent the mean ± SEM (n = 3), *p < 0.05; **p < 0.01, ***p < 0.001. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)