Primer and probe working stock
| Reagent | Final concentration | Amount |
|---|---|---|
| ddH2O | n/a | 85 μL |
| Fwd primer | 16.6 μM | 5 μL |
| Rev primer | 16.6 μM | 5 μL |
| Probe | 5 μM | 5 μL |
| Total | n/a | 100 μL |
Store at −20°C for two years
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