| PCR cycling conditions | |||
|---|---|---|---|
| Steps | Temperature | Time | Cycles |
| Initial enzyme activation | 95°C | 10 m | 1 |
| Denature | 94°C | 30 s | 60 cycles |
| Anneal and extend | 60°C | 60 s | |
| Enzyme deactivation | 98°C | 10 m | 1 |
| Hold | 4°C | forever | |
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