REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
PerCP-conjugated anti-HA tag antibody	Abcam	Cat# ab1117491
APC-conjugated anti-human IgG Fc	Biolegend	Cat# 410712 RRID:AB_2565790
anti-HA-tag antibody	EasyBio	Cat# BE2007
Goat anti-mouse IgG(H+L)-HRP	Gene-Protein Link	Cat# P03S01M
HRP-conjugated goat anti-human IgG(H+L)	Proteintech	Cat# SA00001-17 RRID:AB_2890979
anti-His-tag mAB-HRP-DirecT A	MBL	Cat# D291-7; RRID: AB_10694870
Bacterial and virus strains
Escherichia coli (E.coli) strain T1	TransGen	Cat# CD501-2
Escherichia coli (E.coli) strain DH10Bac	BioMed	BC112
Escherichia coli (E. coli) strain BL21 (DE3)	Novagen	Cat# 69450
Chemicals, peptides, and recombinant proteins
BS3 (bis (sulfosuccinimidyl) suberate)	Thermo	Cat# 21580
EndoH	This paper	N/A
PNGase	This paper	N/A
TROP-2-ECD-293T	This paper	N/A
TROP-2-ECD-insect	This paper	N/A
sacituzumab	This paper	N/A
Critical commercial assays
Crystallization kits	Hampton Research and Molecular Dimensions and Wizard Classic	http://www.hamptonresearch.com; https://www.moleculardimensions.com/;https://rigakureagents.com/
HisTrap excel 5mL column	GE Healthcare	Cat# 17371205
RESOURCETM S 6 mL column	GE Healthcare	Cat# 17118001
Hiload Superdex® 200 16/600 pg column	GE Healthcare	Cat# 2898933
Superdex® 200 Increase 10/300 GL column	GE Healthcare	Cat# 28990944
HiTrap Protein A HP	GE Healthcare	Cat# 17040303
Deposited data
TROP-2-ECD-293T	This paper	PDB: 7E5M
TROP-2-ECD-insect	This paper	PDB: 7E5N
Experimental models: cell lines
HEK-293T cells	ATCC	CRL-3216; RRID:CVCL_0063
High Five cells	Invitrogen	B85502
Sf9 cells	Invitrogen	B82501
Recombinant DNA
pFastBac1-TROP-2-ECD-insect	This paper	N/A
pCAGGS	MiaoLingPlasmid	Cat# P0165
pCAGGS-TROP-2-ECD-293T	This paper	N/A
pCAGGS-TROP-2-N33Q	This paper	N/A
pCAGGS-TROP-2-N120Q	This paper	N/A
pCAGGS-TROP-2-N168Q	This paper	N/A
pCAGGS-TROP-2-N208Q	This paper	N/A
pCAGGS-TROP-2-WT	This paper	N/A
pCAGGS-mTROP-2	This paper	N/A
pCAGGS-TROP-2-mCRD	This paper	N/A
pCAGGS-TROP-2-mTY	This paper	N/A
pCAGGS-TROP-2-mCPD	This paper	N/A
pCAGGS-TROP-2-mRCPD	This paper	N/A
pCAGGS-TROP-2-mACPD	This paper	N/A
pCAGGS- sacituzumab-H-chain	This paper	N/A
pCAGGS- sacituzumab-L-chain	This paper	N/A
pET28a-PNGase	This paper	N/A
pET28a-ENdoH	This paper	N/A
Software and algorithms
PyMOL software	Molecular Graphics System, Version 1.8 Schrö dinger	https://pymol.org/2/ RRID:SCR_000305
FlowJo 7.6.1	FLOWJO	https://www.flowjo.com/solutions/flowjo/downloads
Phenix	Adams et al., 2010	http://www2.mrclmb.cam.ac.uk/Personal/pemsley/coot/; RRID:SCR_014222
Coot	Emsley and Cowtan, 2004	http://www.phenixonline.org/; RRID:SCR_014224
CCP4	Collaborative Computational Project	https://www.ccp4.ac.uk/ RRID:SCR_007255
MolProbity	Chen et al., 2010	N/A
Origin	OriginLab	https://www.originlab.com/ RRID:SCR_002815