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. 2021 Oct 1;11:743050. doi: 10.3389/fonc.2021.743050

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Copyright © 2021 Chen, Huang, Yang, Chen, Shi, Li, Chen and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mCALR silence inhibits the migration and maturation of DCs by decreasing the secretion of TNFα and CCL19. (A) The relative mRNA expression levels of CALR was detected in siCALR and control groups using real-time PCR; ***P < 0.001. (B, C) The effect of cell supernatants (siCtrl and siCALR) on DC migration was evaluated by migration assay; **P < 0.01, ***P < 0.001. (D–G) The effect of cell supernatants (siCtrl and siCALR) on DC maturation was determined using flow cytometry with PE-anti-human CD83 antibody; **P < 0.01, ***P < 0.001. (H, I) The relative mRNA expression levels of TNFα and CCL19 were detected in siCALR and control groups using real-time PCR; **P < 0.01, ***P < 0.001. (J, K) The expression levels of CALR, Flag-mCALR, TNFα, and CCL19 were investigated in siCALR and control groups using western blot; *P < 0.05, **P < 0.01, ***P < 0.001.