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. 2021 Oct 1;12:728685. doi: 10.3389/fimmu.2021.728685

Figure 1.

Figure 1

Effect of LLT1 expression on MAIT cell function. B-LCLs were infected with S. Typhi for 3 hours. Uninfected cells were used as controls. After 16-18 hours of gentamicin treatment, cells were stained with anti-LLT1 Ab and analyzed by flow cytometry. (A) Percentage of LLT1 on the surface of B-LCL cells. Cumulative LLT1 (B) percentage and (C) mean fluorescence intensity (MFI). Bar graphs extend from the 25th to 75th percentiles; the line in the middle represents the median of the pooled data. The whiskers delineate the smallest to the largest value. (D) S. Typhi infection levels in live LLT-1- and LLT1+ B-LCLs. (E) real-time RT-PCR for LLT-1. Data are representative of one independent experiment with 4 volunteers, three replicates each. (F) Representative gating strategy. (G) PBMC were exposed to either uninfected (UN) or S. Typhi-infected (INF) B-LCLs treated with different concentrations of Abs to LLT1 (LLT1) or isotype (ISO) control (5 and 20 μg/ml). After 16-18 hours of co-culture, cells were collected, and the levels of IFN-γ expressing MAIT cells were evaluated by flow cytometry. (H) Cumulative data of IFN-γ + MAIT cells after LLT1 blocking. Data are representative of two independent experiments.