Skip to main content
. 2021 Oct 1;12:728685. doi: 10.3389/fimmu.2021.728685

Figure 5.

Figure 5

Inhibitory HLA-G receptor CD85j and IFN-γ secretion by MAIT cells. PBMC were exposed to either uninfected (UN) or S. Typhi-infected (INF) B-LCL cells treated with Abs to HLA-G (HLA-G) or isotype (ISO) control (2.5 μg/ml). After 16-18 hours of co-culture, cells were collected, and the levels of MAIT cells expressing CD85j and IFN-γ were evaluated by flow cytometry. To gate on MAIT cells, a “dump” channel was used to eliminate dead cells (ViViD+), as well as macrophages/monocytes (CD14+) and B-LCLs (CD45+) from the analysis. (A) Representative experiment showing CD85j expression on MAIT cells. (B) Cumulative data. Levels of MAIT cells expressing (C) CD85j only or co-expressing (D) IFN-γ after HLA-G blocking. (E) Kinetics of CD85j co-expression on MAIT cells after exposure to UN or INF B-LCLs. Student’s t-test was performed to compare CD85j expression on MAIT cells after exposure to UN or INF B-LCLs. *, represent significant differences (P<0.05) between MAIT cells exposed to UN (○) or INF B-LCLs (•) at the same timepoint. (F) Pearson’s correlations among levels of MAIT cells and their expression of CD85j and IFN-γ, and the expression of CSA and IL-10 on B-LCLs. Each dot represents a single experimental condition. Data are representative of one out of 2 individual experiments. Bar graphs extend from the 25th to 75th percentiles; the line in the middle represents the median of the pooled data. The whiskers delineate the smallest to the largest value.