Table 2.
Effects of the β-glucosidase on different substrates.
| Substrates | Relative activity (%) | Km (mm−1) | Vm (μmol/min/mgzen) | kcat (s−1) | kcat/Km (mm−1s−1) |
|---|---|---|---|---|---|
| Chromogenic substrate (0.5mm) | |||||
| p-Nitrophenyl β-D-glucopyranoside (pNPGlc) | 99.7 ± 1.8 | 0.617 | 139.2 | 135.1 | 220.5 |
| p-Nitrophenyl-β-D-galactopyranoside (pNPGal) | 75.4 ± 3.1 | 1.054 | 127.4 | 124.5 | 118.1 |
| Oligosaccharides (5mm) | |||||
| Cellobiose (β-1,4) | 100.5 ± 8.9 | 6.24 | 24.3 | 23.8 | 3.81 |
| Lactose (β-1,4) | 52.6 ± 6.5 | 38.1 | 7.59 | 7.46 | 0.196 |
| Maltose (α-1,4) | ND | ||||
| Sucrose (α-1,2) | ND | ||||
| CMC (1%) | ND | ||||
Values presented are the means ± SD, and the SD was calculated from three independent reaction tubes using the same purified BGL