FIGURE 5.

Characterization of the antigen storage compartments in DCs upon MGL1 targeting. DCs were pulsed with OVA‐Le^X for 2 h, washed and chased for 2, 24, 48 or 72 h. CFSE‐labelled OTI CD8⁺ T cells were added, and T‐cell proliferation read‐out was analysed by flow cytometry (a). DCs were pulsed with OVA‐Le^X (DyLight 488) for 15 min or pulsed with OVA‐Le^X for 2 h and chased for 48 h. OVA‐Le^X presence in DCs was imaged by confocal microscopy and specific antibodies against EEA1, Rab5, Rab7, LAMP1, MHCI, MHCII, TAP1 and PA28β (red) were used and analysed for co‐localization with OVA‐Le^X (green). Co‐localization between OVA‐Le^X and the antibodies is summarized in a table, ‘+’ indicates co‐localization, and ‘−’ indicates no co‐localization (b)