FIGURE 6.

Antigens targeted to FcγRs and MGL1 on DCs end up in the same compartments. DCs were pulsed with OVA IC (Alexa Fluor 647) or OVA‐Le^X (DyLight 488) for 15 min and 1 h, or pulsed for 2 h and chased for 24 or 48 h. Co‐localization between OVA IC (red) and OVA‐Le^X (green) was visualized by confocal microscopy and DIC was used to image cell contrast. Histograms for each fluorophore were created for a selected area (indicated by a line on the image) and overlays were made with the ImageJ software. Arrows indicate co‐localization between OVA IC (red) and OVA‐Le^X (green)