FIGURE 1.

MON‐DNJ reduces DENV‐ and LPS‐induced inflammation in macrophages. (a–i) Nine of twelve cytokines assayed on a Luminex bead‐based platform at 24 h p.i. demonstrate differential expression in macrophages treated with media (UI), DENV 2 16681 at moi =1 (DENV) or LPS from S. enterica at 100 ng/ml (LPS). Equivalent data were collected and analysed at 72 and 120 h p.i. (Figure S2). MON‐DNJ samples (grey bars) were compared to untreated samples (black bars) by one‐way, repeated‐measures ANOVA or equivalent ANOVA of ranks for non‐normally distributed data with post hoc pairwise comparison using the Holm–Šídák method (parametric) or Tukey test (non‐parametric). Induction of cytokine by each mode of infection was tested independently by ANOVA with correction for multiple comparisons across time points using Holm–Šídák method. Biological replicates (n = 5) were assayed in technical singlicate. Discontinuous axes are used where necessary as a consequence of >10‐fold difference in level of cytokine induced by DENV and LPS. Samples were normalized to untreated controls for individual donor and stimulus and evaluated by parametric t‐testing with Holm–Šídák correction for multiple testing. *p < 0·05, **p < 0·01, ***p < 0·001