Table 4.
Percentage of vit. D2 retention at different pH and thermal treatment at 75 ℃ incubated at different time points in the dark. Number of repetition for each treatment: n = 3. The data are represented as the mean ± S.D. from 3 independent experiments where *p < 0.05, **p < 0.01, ***p < 0.001, vit. D2 (5 μg) vs vit. D2 (5 μg) + quercetin (5 μg), vit. D2 vs vit. D2 (5 μg) + quercetin (10 μg). Statistical significance analysis was carried out through one-way analysis of variance (ANOVA) prism.
| Vitamin and Quercetin | pH = 1, 75 °C, 2 h(% of vit. D2 retention) | pH = 4, 75 °C , 2 h(% of vit. D2 retention) | pH = 5, 75 °C , 2 h(% of vit. D2 retention) | pH = 7, 75 °C , 2 h(% of vit. D2 retention) | pH = 10, 75 °C , 2 h(% of vit. D2 retention) |
|---|---|---|---|---|---|
| Vit D2 (5 μg) | 28.59 ± 0.48 | 65.77 ± 2.96 | 62.44 ± 2.88 | 46.46 ± 6 | 45.42 ± 5 |
| Vit D2 (5 μg) +Quercetin(5 μg) | 96..04 ± 5.19 | 93.96 ± 4.16 | 91.96 ± 1.73 | 85.73 ± 5 | 92.45 ± 1.52 |
| Vit D2 (5 μg) +Quercetin (10μg) | 97.96 ± 3.46 | 95.90 ± 4 | 96.56 ± 3.05 | 91.2 ± 5 | 97.94 ± 2.88 |