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. 2021 Oct 5;11(19):e4175. doi: 10.21769/BioProtoc.4175

Figure 1. Schematic of mouse classical pathway (CP) CH50 haemolytic assays.

Figure 1.

Top panel: diagram of modified CP CH50 haemolytic assay utilising double sensitised ShEA and high amounts of mouse serum (typically 25% male, 50% female). Bottom panel: diagram of the mouse component-specific CP CH50 haemolytic assay utilising 10% human serum depleted of the component of interest and different amounts of mouse serum as source of the component. MAC, membrane attack complex; ShEA, sheep erythrocytes sensitised with amboceptor.