Figure 1. Schematic of mouse classical pathway (CP) CH50 haemolytic assays.
Top panel: diagram of modified CP CH50 haemolytic assay utilising double sensitised ShEA and high amounts of mouse serum (typically 25% male, 50% female). Bottom panel: diagram of the mouse component-specific CP CH50 haemolytic assay utilising 10% human serum depleted of the component of interest and different amounts of mouse serum as source of the component. MAC, membrane attack complex; ShEA, sheep erythrocytes sensitised with amboceptor.