Skip to main content
. 2021 Sep 25;24(10):103173. doi: 10.1016/j.isci.2021.103173

Figure 2.

Figure 2

Baseline SLFN11 expression in JAK GOF mutant leukemia cells is independent of the classical IFN-STAT pathway and SLFN11 induction by IFN is cell line-dependent

(A–C) MOLT4 (A), CCRF-CEM (B) and HEL (C) cells were treated with IFN-α (10,000 U/mL) or IFN-γ (100 ng/mL) for 6 or 24 h.

(D) Relative expression profiling of SLFN11 in multiple cell lines in the response to IFN-α (10,000 U/mL) or IFN-γ (100 ng/mL) for 6 or 24 h. The original images of Western blotting were listed in Figure S2C.

(E) Transcriptional induction of SLFN11 mRNA by IFNs in CCRF-CEM, MOLT4, and HEL cells. Relative 2−ΔΔCT changes of SLFN11 mRNA expression following treatment with IFN-α (10,000 U/mL) or IFN-γ (100 ng/mL) for 6 or 24 h in MOLT4 (N = 2), CCRF-CEM (N = 2) and HEL (N = 2). Non-small-cell lung cancer A549 cells were used as an IFN-inducible control cells (N = 3). Error bars represent SD; ∗P = 0.03, ∗∗∗P < 0.001 with ordinary one-way ANOVA test and Tukey's multiple comparisons test.