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. 2021 Sep 25;24(10):103173. doi: 10.1016/j.isci.2021.103173

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Inhibitors of JAK pathway decrease SLFN11 expression

(A and B) SLFN11 and phosphorylated TYK2 expression detected by Western blotting after cerdulatinib treatment. MOLT4 was treated with cerdulatinib (40 μM) for the indicated times. Quantitation of SLFN11 and phosphorylated TYK2 by 3 independent experiments as shown in panel A. Error bars represent SD; ∗p = 0.032, ∗∗p = 0.008, ∗∗∗p < 0.001 (SLFN11), ^†††p < 0.001 (p-TYK2) with ordinary one-way ANOVA test and Tukey's multiple comparisons test.

(C and D) SLFN11 and phosphorylated TYK2 expression in CCRF-CEM treated with cerdulatinib (40 μM). Quantitation of SLFN11 and phosphorylated TYK2 in 3 independent experiments as shown in panel D. Error bars represent SD; ∗p = 0.013, ∗∗p = 0.003, ∗∗∗p < 0.001 (SLFN11), ^†††p < 0.001 (p-TYK2) with ordinary one-way ANOVA test and Tukey’s multiple comparisons test.

(E) Relative changes of SLFN11 mRNA expression treated with cerdulatinib (40 μM) for the indicated times in MOLT4 and CCRF-CEM cells (N = 3). Error bars represent SD; ∗∗∗p < 0.001 with two-way ANOVA test and Tukey’s multiple comparisons test.

(F) Restoration of SLFN11 expression after release of cerdulatinib treatment (40 μM, 24 h) in MOLT4 cells.

(G) SLFN11 and phosphorylated TYK2, JAK1, JAK2, and STAT1 expression detected by Western blotting in HEL cells treated with cerdulatinib (40 μM) for the indicated times.

(H) Restoration of SLFN11 expression in HEL cells after release of cerdulatinib treatment (40 μM, 24 h).

(I) Quantitation of the relative SLFN11 protein expression in 2 independent experiments as shown in Figures S3A, S3C, and S3D. Error bars represent SD; ∗p = 0.039, ∗∗∗p < 0.001 with two-way ANOVA test and Tukey’s multiple comparisons test.