REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-TYK2	Cell signaling Technology	Cat# 9312S; RRID: AB_2256719
Rabbit monoclonal anti-phospho (Y1054/1055)-TYK2 (D7T8A)	Cell signaling Technology	Cat# 68790S; RRID: AB_2799752
Rabbit monoclonal anti-phospho (Y1034/1035)-JAK1 (D7N4Z)	Cell signaling Technology	Cat# 74129S; RRID: AB_2799851
Rabbit polyclonal anti-phospho (Y1007/1008)-JAK2	Cell signaling Technology	Cat# 3771S; RRID: AB_330403
Rabbit monoclonal anti-phospho (Y701)-STAT1 (58D6)	Cell signaling Technology	Cat# 9167S; RRID: AB_561284
Rabbit polyclonal anti-phospho (T202/Y204)-p44/42 MAPK (ERK1/2)	Cell signaling Technology	Cat# 9101S; RRID: AB_331646
Rabbit polyclonal anti-ETS-1 (C-20)	Santa Cruz Biotechnology	Cat# sc-350; RRID: AB_2100688
Rabbit polyclonal anti-FLI-1 (C-19)	Santa Cruz Biotechnology	Cat# sc-356; RRID: AB_2106116
Mouse monoclonal anti-ISG15 (F-9)	Santa Cruz Biotechnology	Cat# sc-166755; RRID: AB_2126308
Mouse monoclonal anti-SLFN11 (D-2)	Santa Cruz Biotechnology	Cat# sc-515071
Rabbit polyclonal anti-GAPDH	GeneTex	Cat# GTX100118; RRID: AB_1080976
ECL anti-mouse IgG, horseradish peroxidase linked whole antibody (from sheep)	GE Healthcare	cat# NA931; RRID: AB_772210
ECL anti-rabbit IgG, horseradish peroxidase linked whole antibody (from donkey)	GE Healthcare	cat# NA934; RRID: AB_772206
Chemicals, peptides, and recombinant proteins
Recombinant human interferon-gamma	Thermo Fisher Scientific	Cat# PHC4031
Human interferon-alpha a protein (alpha 2a)	PBL assay science	Cat# 11101-2
Cerdulatinib	MedChemExpress	Cat# HY-15999
Ruxolitinib	MedChemExpress	Cat# HY-50856
Tofacitinib	MedChemExpress	Cat# HY-40354
MK-2206 dihydrochloride	MedChemExpress	Cat# HY-10358
SCH772984	MedChemExpress	Cat# HY-50846
TK216	Shelleckchem	Cat# S9718
LY294002 (InSolution)	Sigma-Aldrich	Cat# 440204
Retinoic acid	Sigma-Aldrich	Cat# R2625
Critical commercial assays
TRIzol reagent	Invitrogen	Cat# 15596026
PureLink RNA Mini Kit	Invitrogen	Cat# 12183025
SuperScript II Reverse Transcriptase Kit	Invitrogen	Cat# 18064022
FastStart Universal SYBR Green Master (Rox)	Sigma-Aldrich	Cat# 4913850001
protease inhibitor cocktail (100x)	Cell signaling Technology	Cat# 5871S
Pierce phosphatase inhibitor mini tablets	Thermo Fisher Scientific	Cat# A32957
Novex tris-glycine SDS sample buffer	Invitrogen	Cat# LC2676
Tris/glycine/SDS buffer (10x)	BioRad	Cat# 1610732
Novex tris-glycine transfer buffer (25x)	Invitrogen	Cat# LC3675
Blotting-Grade Blocker	BioRad	Cat# 1706404
Immun-Blot PVDF membranes	BioRad	Cat# 1620177
Experimental models: Cell lines
Human: prostate cancer DU145 cells	Developmental Therapeutics Program (NCI/NIH)	N/A
Human: colon cancer HCT116 cells	Developmental Therapeutics Program (NCI/NIH)	N/A
Human: leukemia CCRF-CEM cells	Developmental Therapeutics Program (NCI/NIH)	N/A
Human: leukemia MOLT4 cells	Developmental Therapeutics Program (NCI/NIH)	N/A
Human: leukemia K562 cells	Developmental Therapeutics Program (NCI/NIH)	N/A
Human: leukemia JURKAT cells	Developmental Therapeutics Program (NCI/NIH)	N/A
Human: leukemia THP-1 cells	Developmental Therapeutics Program (NCI/NIH)	N/A
Human: leukemia HL-60 cells	Dr. T. Breitman NCI, NIH	N/A
Human: leukemia HAP-1 cells	Horizon Discovery	N/A
Human: lung cancer A549 cells	Developmental Therapeutics Program (NCI/NIH)	N/A
Human: lung cancer DMS114 cells	American Type Culture Collection	Cat# CRL-2066
Human: leukemia HEL cell	Dr. Peter D. Aplan NCI, NIH	N/A
CCRF-CEM SLFN11 knockout cells	Murai et al., 2016	N/A
MOLT4 SLFN11 knockout cells	Murai et al., 2016	N/A
Oligonucleotides
Forward primer for qPCR of SLFN11: 5′-GGCCCAGACCAAGCCTTAAT-3′	IDT oligo	N/A
Reverse primer for qPCR of SLFN11: 5′-CACTGAAAGCCAGGGCAAAC-3′	IDT oligo	N/A
Forward primer for qPCR of ETS-1: 5′-GTTAATGGAGTCAACCCAGC-3′	IDT oligo	N/A
Reverse primer for qPCR of ETS-1: 5′-GGGTGACGACTTCTTGTTTG-3′	IDT oligo	N/A
Forward primer for qPCR of GAPDH: 5′-TCAACGACCACTTTGTCAAGCT-3′	IDT oligo	N/A
Reverse primer for qPCR of GAPDH: 5′-GTGAGGGTCTCTCTCTTCCTCTTGT-3′	IDT oligo	N/A
Software and algorithms
GraphPad Prism 9 (software for drawing graphs and statistics analysis)	GraphPad	N/A
ImageJ (software for image analysis)	NIH	N/A
Image Lab software (software for image analysis)	BioRad	N/A
CellMinerCDB (web application for analysis of NCI-60, CCLE and GDSC database)	Genomics & Bioinformatics Group/Developmental Therapeutics Branch/Laboratory of Molecular Pharmacology/CCR/NCI/NIH	https://discover.nci.nih.gov/rsconnect/cellminercdb/
cBioPortal (web application for analysis of TCGA database)	Cerami et al. (2012) and Gao et al. (2013)	https://www.cbioportal.org
Other
QuantStudio 5 Real-Time PCR System	Thermo Fisher Scientific	N/A
ChemiDocTM Touch MP	BioRad	N/A