Table 2.

Operating conditions and mobile phases employed for the quantification of CPX and DOX using HPLC.

Analyte	Mobile phase	Flow type	Flow rate (mL min−1)	Column average temperature (°C)	Injection volume (mL)	Mobile phase relation	Retention time (min)
Cephalexin	A: Water (0.1% v/v formic acid)	Gradient	0.55	35.0 ± 1.0	50.0	A:B 90:10 for 4 min, then A:B 30:70 for 1 min and finally, A:B 90:10 for 4 min.	∼5.45
	B: Acetonitrile
Doxycycline	A: Acetonitrile	Isocratic	0.80	25.0 ± 1.0	50.0	A:B:C 25:55:20 for 5 min.	∼2.25
	B: Water (0.5% v/v acetic acid)
	C: Methanol