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. 2021 Sep 3;100(12):101454. doi: 10.1016/j.psj.2021.101454

Figure 6.

Figure 6

The YAP1 transduction affects the LATS2-induced inhibition in granulosa cell (GC) proliferation. GCs were transfected using the reconstructed pYr-adshuttle-4-YAP1 vector (OE group), pYr-adshuttle-4 empty vector (negative control), and no plasmid (blank control) for 24 h. (A) The expression of YAP1 mRNA with or without transfection of the pYr-adshuttle-4-YAP1 vector by RT-qPCR. (B) Expression levels of YAP1 protein with or without transfection of the pYr-adshuttle-4-YAP1 vector by western blotting. The β-actin was used as the loading control. (C) Abundances of FSHR, STAR, CYP11A1, ESR1, and ESR2 mRNA expression affected by YAP1 overexpression in the GCs. (D) The effects of YAP1 overexpression on granulosa cell proliferation. Scale bars: 50 μm. (E–J) Knockdown of YAP1 abolished the inhibitions of LATS2 on GC proliferation and the mRNA expression levels of FSHR, STAR, CYP11A1, ESR1, and ESR2. NC, the granulosa cells were transfected with pYr-adshuttle-4 empty vector; OE, the cells were transfected with reconstructed pYr-adshuttle-4-LATS2. For each group, bars with superscript symbol shows significant difference compared to the control group ** P < 0.01, * P < 0.05.