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. Author manuscript; available in PMC: 2021 Oct 15.
Published in final edited form as: Nat Genet. 2020 Sep 14;52(10):1036–1045. doi: 10.1038/s41588-020-0684-4

Extended Data Fig. 1 |. Flow cytometry gating strategy of TBNK, regulatory T cell, maturation stages of T cell, and dendritic cell antibody panel.

Extended Data Fig. 1 |

TBNK panel. a, Lymphocytes (violet) and granulocytes (blue). b, CD14+ monocytes (light blue). c, HLA DR++CD14+ monocytes. d, CD3+ (T cells, purple) and CD3− (green) lymphocytes. e, B and NK cells are CD19+ and CD16/CD56+, respectively. f, HLA DR+ NK cells. g, T cells are divided based on CD4 and CD8 expression. h, TCR-ϒδ+ T cells. i, NKT are CD3+ and CD16/CD56+. j, HLA DR+ T cells. HLA DR+CD4 and HLA DR+CD8 subsets are obtained by intersecting HLA DR+ T cell with CD4+ and CD8br lymphocytes. Regulatory T cell panel. a, CD4+ (blue) and CD8+ (violet) lymphocytes. b, CD4+ Tregs (green) are CD25high CD127low. c, Resting (CD45RA+CD25++, pink), activated (CD45RA-CD25+++, orange) and secreting (CD45RA-CD25++, purple) CD4+ Tregs. D-E) CD25hiCD4+ lymphocytes are divided based on CD45RA expression. F) CD4-CD8− T cells (DN, black) are divided in CD28+ and CD28−. G-H-I-J-K) CD39 expression on Treg subsets, CD4 and CD8br T cells, respectively. L) CD8br cells division based on CD45RA vs CD28 expression. M-N) CD25++CD28-CD8br and CD127-CD28-CD8br identification. Maturation stages of T cell panel. a, CD4+ (blue), CD8br (violet) and CD4-CD8− (black) T cells are analyzed for CD45RA vs CCR7 (plots B-C-D, respectively) identifying naïve (CCR7+CD45RA+), central memory (CM, CCR7+CD45RA−), effector memory (EM, CCR7-CD45RA−), and terminally differentiated (TD, CCR7-CD45RA+) subsets. Dendritic cell antibody panel. a, Monocytes (pink). b, c, DCs are Lineage (Lin) negative and HLA DR+. d, Myeloid (green) and plasmacytoid (violet) DCs are CD11c+ and CD123+, respectively. e, f, CD86 and CD62L expression on cDC. G-H-I) CD11c, CD62L and HLA DR expression on monocytes.