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. Author manuscript; available in PMC: 2021 Oct 15.
Published in final edited form as: Nat Genet. 2020 Sep 14;52(10):1036–1045. doi: 10.1038/s41588-020-0684-4

Extended Data Fig. 2 |. Flow cytometry gating strategy of B cell, monocyte, myeloid cell antibody panel.

Extended Data Fig. 2 |

B cell panel. a-b, Lymphocyte (red). c, B lymphocytes (violet) are CD19+. d, IgD+ B cells. B cells classification based on e) CD24 vs CD38; f) CD27 vs IgD; g) IgD vs CD38; h) IgD vs CD24; i) CD24+CD27+ memory B cells. j, Plasma blasts/plasma cells (PB/PC) are CD20-CD38hi B cells. Monocyte panel. A-B-C) Monocyte (blue). D) Monocytes division into CD14+CD16− (classical), CD14-CD16+ (non-classical) and CD14+CD16+ (intermediate). Myeloid panel. A-B-C) Lympho-monocytes (red). d, Viable and myeloid-enriched cells (green) are obtained excluding lymphoid cells, which are lineage1 (Lin1) positive, and dead cells, which are 7-aminoactinomycin-D (7AAD) positive. e, Hematopoietic stem cells (HSC). f, CD14 vs CD66b expression and g) CD33 vs HLA DR expression on myeloid-enriched cells. The intersection of CD33dim/br HLA DRdim/− cells in g) with CD14+ monocytes (orange) in f) results in monocytic myeloid-derived dendritic cells (Mo MDSC). h, The deletion of CD14+ monocytes (orange) from cells in g) discriminates five subsets using CD33 vs HLA DR markers. i, CD66b+ cells were excluded from the CD33dim HLA DR− cells (blue) and j) the resulting CD33dimHLA DR-CD66− population was further divided into basophils and immature MDSC (Im MDSC) based on CD45 and CD11b expression. k, CD33br HLA DR+ cells (black) division into CD14 dim and CD14−. l, CD11b expression on CD33dim HLA DR+ cells (purple). Intersection of CD33dim HLA DR− in h) with CD66b++ cells in f) corresponds to granulocytic myeloid-derived dendritic cells (Gr MDSC).