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. 2021 Sep 13;12(22):6610–6619. doi: 10.7150/jca.58500

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FIN56 induces LMP in a TFEB dependent manner. (A) Reverse transcription PCR of LN229 and U118 cells transfected with shControl or shTFEB. (B) Western blot analysis of LN229 and U118 cells transfected with shControl or shTFEB. (C) Fluorescence images of EGFP-Gal3 transfection of LN229 and U118 cells treated with shControl combined with 1 μM FIN56, shTFEB combined with 1 μM FIN56, shControl combined with 5 μM siramesine (SM), shTFEB combined with 5 μM SM. (D) Quantification of green puncta in (C). (E) Images of CellRox green staining of LN229 and U118 cells treated with shControl combined with 5 μM siramesine (SM), shTFEB combined with 5 μM SM. **P < 0.01; ***P < 0.001; size bars: 10 µm.