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. 2021 Sep 21;12(22):6727–6739. doi: 10.7150/jca.60208

Figure 4.

Figure 4

Metformin reduced the expression of Mcl-1 in KG-1 and THP-1 cells by inhibiting protein production, and also decreased the transcription level of Mcl-1 in THP-1. The transcription level of Mcl-1 (A) and Bcl-2 (B) were measured after treatment with metformin for 48 hours in KG-1, Kasumi-1 and THP-1 cells. The level of Mcl-1, Bcl-2, Bcl-xl proteins were detected by western blot after cycloheximide, metformin, cycloheximide/metformin treatment for 48 hours in THP-1 (C) and KG-1 (E). The time-course degradation of Mcl-1 protein in the figure (C) and figure (E) graph were plotted in (D) and (F). The results are shown as bar graphs with the mean± s.d.,n =3 independent experiments. The statistical significance was determined by Student's t-test. The level of Mcl-1, Bcl-2, Bcl-xl proteins were detected by western blot after metformin, MG-132, metformin/MG-132 treatment for 48 hours in THP-1 (G) and KG-1 (H).