FIG. 7.
CM from ERK5‐silenced cells less effectively recruits myofibroblasts and monocytic cells. (A,B) CM was collected from 6 × 105 shNT or shERK5 HuCCT‐1 and CCLP‐1 cells, incubated in complete medium for 24 hours, and used for migration assay. Twenty‐four–hour starved HSCs, THP‐1, and PMA‐derived THP‐1 cells were incubated in presence or absence of 10% FBS or different CMs, as indicated. The number of migrated cells is represented as fold increase over control. *P ≤ 0.05 vs. control, **P ≤ 0.05 vs. 10% FBS, ***P ≤ 0.05 vs. shNT CM (n = 3). Abbreviation: PMA, phorbol 12‐myristate 13‐acetate.