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Protocols for phenotyping root hairs in a large number of plants. (a) Imbibed wheat seeds are aligned in a row on moist germination paper placed on top of a tray; or (b) the paper can be rolled and inserted into cones. (c) After 10 d of vertical growth, root hairs are ready to be imaged with a stereomicroscope. (d) Representative images of unstained wheat root hairs for length and density measurements. Bar, 500 µm.
