Fig. 31.

Protocol for preparing Arabidopsis seedlings for live root hair imaging. (a) All material for handling seeds is sterilised and prepared in a laminar flow hood. (b, c) Sterilised seeds are picked individually with a pointed toothpick and planted on nutrient‐supplemented gel layered onto a coverslips. (d) Coverslips with seeds are placed inside Petri dishes and kept at an inclined angle in a growth chamber. (e) After 3–5 d, the coverslips with seedlings can be transferred directly onto a stage of a microscope for image acquisition.