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. 2021 Oct 5;232(3):973–1122. doi: 10.1111/nph.17572

Table 3.

Recommended storage methods of washed roots for further root trait measurements.

Storage method Acceptable storage period Biomass Chemistry Morphology Mycorrhizal colonisation Anatomy
Oven drying c. 60°C > 1 yr √ (total C, N, P, etc.)
Freeze drying > 1 yr √ (labile/volatile; carbohydrates)
+4°C < 2 d
−20°C < 1 yr
−80°C > 1 yr
Ethanol : water 60–95% : 40–5% v/v < 2–4 wk at 20°C; < 1 yr at 4°C
Glutaraldehyde : formaldehyde : cacodylate, 2% : 2% : 96% v/v