Fig. 1.

Overview of three sequencing methods utilized to study APA. a Schematic of sequencing methods—RNA-Seq, 3′-Seq, and Iso-Seq—that facilitate the study of APA. Examples of RNA-Seq methods to study APA. b Metagene plots showing read coverage centered around the transcription termination site (TTS) for five RNA-Seq libraries, five 3′-Seq libraries, and eight Iso-Seq libraries collected from YRI LCLs