Fig. 2.

Library preparation from cfDNA. A TACS-T4 scheme for library preparation from ssDNA. For details, see Figure S3A. B Efficiency of each step of TACS-T4 scheme applied to a model ODN. C–G The size distribution of reads for five different cfDNA libraries. The cfDNAs used for the library preparations were purified with the PPIP method (C, E, F, and G) and QIAamp Circulating Nucleic Acid Kit (D). The PPIP purified DNA was treated with (E) or without (C, D, F, and G) a mixture of E. coli exonuclease I and Rec J before the library preparation. The heat denaturation before library preparation was omitted for G. The libraries were prepared with TACS-T4 (C, D, E, and G) or a commercially available kit (F, ThruPLEX DNA-Seq kit, Takara Bio Inc.). For details, see Supplementary Methods