Table 2.
Comparative cell growth and volumetric production (protein titre) during recombinant disulphide-bond protein production in the wild-type (wt) and ΔdsbB strain
| Proteins | Cell growtha and protein titreb | |
|---|---|---|
| Strains | ||
| Wild-type (wt) | ΔdsbB | |
| scFv13R4 | 5.04 ± 0.14 | 3.27 ± 0.08 |
| 6.25 ± 0.73 mg/L | 1.16 ± 0.42 mg/L | |
| scFv13R4CM | 5.40 ± 0.20 | 3.68 ± 0.00 |
| 9.48 ± 1.00 mg/L | 3.50 ± 0.23 mg/L | |
| RNase A | 2.96 ± 0.08 | 3.05 ± 0.05 |
| 3.15 ± 0.14 mg/L | 0.26 ± 0.06 mg/L | |
| NGRP | 4.43 ± 0.06 | 4.43 ± 0.11 |
| 13.11 ± 2.25 mg/L | 11.12 ± 1.93 mg/L | |
aFinal cell density was measured as Abs600 recorded 4 h after induction
bTotal protein titre was determined from the Western blot quantification data (glycosylated and non-glycosylated), normalised and converted from sample OD600 of periplasmic fraction and sample volume for Western blot. For the final cell density and protein titre, the average of three biological replicates is shown and the errors indicate standard deviation. Cell culture was performed under standard cultivation condition in shake flask 10:50 ml. The change (increasing or decreasing) of cell growth and protein titre relative to control (production in wt strain) was analysed by t-test with Welch’s correction (Additional file 2: Table S4)