Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 14;14:136. doi: 10.1186/s13048-021-00888-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — MiR-136 bound to CBX2 in PTX-resistant SKOV3 and HeyA8 cells. (A) Starbase online database was employed to predict the binding sites between miR-136 and CBX2. (B and C) Luciferase activity was detected by dual-luciferase reporter assay in PTX-resistant SKOV3 and HeyA8 cells. (D-G) The mRNA and protein expressions of CBX2 were detected by qRT-PCR and western blot in PTX-resistant ovarian cancer tissues (N = 20), PTX-sensitive ovarian cancer tissues (N = 19), SKOV3 and HeyA8 cells as well as PTX-resistant SKOV3 and HeyA8 cells. (H and I) The effects of circ_0061140 knockdown on the mRNA and protein expressions of CBX2 were demonstrated by qRT-PCR and western blot, respectively, in PTX-resistant SKOV3 and HeyA8 cells. (J) Spearman correlation analysis was conducted to elucidate the linear relationship between CBX2 and circ_0061140 expressions. (K-M) The influences between miR-136 mimic and CBX2 on the mRNA and protein expressions of CBX2 were demonstrated by qRT-PCR and western blot, respectively, in PTX-resistant SKOV3 and HeyA8 cells. *P < 0.05