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. 2021 Oct 15;12:541. doi: 10.1186/s13287-021-02604-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization of ERCs and upregulation of Gal-9 in ERCs. A Cell surface markers of ERCs were measured by flow cytometry. ERCs express CD29, CD44, CD90, while lacking of CD45, CD31. B Cell morphology images (magnification 40×) of ERCs at P2-5. C Brightfield and fluorescence pictures are shown on the left and right side. Representative picture of GFP-Gal-9-LVs transfected ERCs (a-b) and unmodified ERCs(c-d) (magnification 40×). D Gal-9 protein expression in unmodified ERCs and GFP-Gal-9-LVs transfected ERCs. n = 3. **p < 0.01. E Gal-9 mRNA relative expression in unmodified ERCs and GFP-Gal-9-LVs transfected ERCs. n = 3. **p < 0.01. p value was calculated using unpaired t-test. Abbreviations: ERCs endometrial regenerative cells, Gal-9 galectin-9, P3-5 passage3-5