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. 2021 Oct 15;12:541. doi: 10.1186/s13287-021-02604-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Gal-9 mediates ERCs to reduce Th1 and Th17 cell differentiation, but improve Treg population in vivo. Single-cell suspensions were obtained from the spleen and liver. Cells were stained with anti-mouse CD4, IFN-γ, IL-17, CD25, Foxp3 antibodies. A Representative dot plots depict the percentage of Tregs (CD4⁺CD25⁺Foxp3⁺) in the spleen and liver. B Representative dot plots depict the percentage of Th1 (CD4⁺IFN-γ⁺) cells in the spleen and liver. C Representative dot plots depict the percentage of Th17 (CD4⁺IL-17⁺) cells in the spleen and liver. D Percentage of Tregs in spleen and liver. E Percentage of Th1 cells in spleen and liver. F Percentage of Th17 cells in spleen and liver. The differences between groups were calculated using ANOVA. *p < 0.05, **p < 0.01. Abbreviations: Treg regulatory T cells, ANOVA one-way analysis of variance