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Nanopore‐based thiamine sensor. A) Representation of the experimental setup with the Cytolysin A (ClyA‐AS, blue) nanopore hosting the thiamine‐binding protein (TbpA, green, PDB: 2QRY) and free thiamine monophosphate. The ClyA‐TbpA sensor pair is embedded within a lipid bilayer (orange) B) Enlarged TbpA with bound thiamine monophosphate (PDB: 2QRY). C) Chemical structure of thiamine and its phosphorylated derivates.
