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. 2021 Jul 5;50(8):795–802. doi: 10.1111/jop.13221

FIGURE 2.

FIGURE 2

Low Annexin A1 expression enhanced cell proliferation by regulating p27 expression. The expression of Annexin A1 mRNA was analysed using real‐time PCR in HB96 (A) and HN4 (E) cells transfected with retroviral vectors containing shRNA1, shRNA2 or shRNAnc. The expression of Annexin A1 in HB96 (B) and HN4 (F) cells transfected with retroviral vectors containing shRNA1, shRNA2 or shRNAnc was assessed by Western blotting. The proliferation of HB96 (C) and HN4 (G) cell lines following shRNA transfection was detected using a CCK‐8 kit at 450 nm. The expression of pEGFR, PDK1, pPDK1, AKT, pAKT and p27 in HB96 (D) and HN4 (H) cells transfected with shRNA retroviral vectors was detected by Western blotting (*p < 0.05, **p < 0.01, ***p < 0.001).