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. 2021 May 5;76(10):3028–3040. doi: 10.1111/all.14832

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© 2021 The Authors. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Multiplex flow cytometry using multiple allergen tetramers in a single tube as a component‐resolved diagnostic test for allergy. A, B) Representative histograms of basophils from thawed PBMC of a control (blue) or an RGP‐allergic patient (red) stained with Lol p 1 and Lol p 5 tetramers in a single tube. C) 2D dot plot of Lol p 1 and Lol p 5 tetramer staining of the basophils from the control and patient in panels A and B. D) Scatterplot depicting MFI ratios of allergen:streptavidin for [Lol p 1]₄‐APC vs strep‐APC and [Lol p 5]₄‐BV711 vs strep‐BV711 on basophils from controls (blue, n = 10) and RGP‐allergic subjects (red, n=15) stained with both allergen tetramers or both streptavidin conjugates in a single tube.