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A) Transient absorption map of NsXeR WT and the corresponding decay associated spectra (D.A.S.) in B) with the fitted lifetimes. Transient absorption of the mutants D220N, D220E and the WT detected at C) 587 nm and D) 648 nm. E) Transient map of NsXeR WT. Transient absorption at 390 nm (F), 470 nm (G) of WT pH 7.4, 5 and 9 and the mutants D220E and D220N at pH 7.4.
