Figure 1.

Evaluation of nanobody–fluorophore conjugates for super‐resolution microscopy. a) The fluorescent, R10‐functionalized GFP‐binding nanobody GBP1 enters cells after which the R10 peptide is cleaved off. b–d) HeLa kyoto cells expressing GFP–PCNA were treated with 2 μm of the GPB1 nanobody variants. Colocalization with GFP and intracellular stability were assessed by confocal microscopy after 1 and 18 hours post‐treatment of the cells. Scale bars 20 μm. e) STED images from the time series of the nanobody–fluorophore conjugates in living cells. Scale bars 5 μm.