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. 2021 May 7;1500(1):34–47. doi: 10.1111/nyas.14601

Figure 5.

Figure 5

S. clandestina aqueous extract inhibits cell migration. Differential cell migration rate was examined using a wound closure assay. Cells were treated or not with 20 μg/mL of S. clandestina extract and monitored by microscopy at the indicated times. (A) Migration rate (average ± SD) and degree of wound closure were assessed by measuring the distance between wound edges at the indicated time intervals in at last eight randomly chosen regions of four different experiments normalized to 100% wound closure for control cells. P < 0.001 by one‐way ANOVA (n = 4); values with shared letters are not significantly different according to Bonferroni/Dunn post hoc test. (B) Cells were treated with the JNK1/2 inhibitor SP600125 or the p38 inhibitor SB203580 for 30 min before stimulation with or without S. clandestina extract. After 24 h, migration was evaluated. Results (means ± SD) from four independent experiments are presented. Migration was expressed as the percentage of unstimulated cells at 24 hours. P < 0.001 by one‐way ANOVA (n = 4); values with shared letters are not significantly different according to Bonferroni/Dunn post hoc tests.