FIGURE 3.

Transduction of human adherent cell lines with fiber‐modified FAdV‐4 vectors. (A) Preliminary experiments were performed with human 293 cells or A549 cells. Cells were infected with fiber modified viruses or FAdV4‐GFP (control) at a MOI (multiplicity of infection) of 1000 vp/cell (viral particle per cell) for 4 hours. Cells were detached for the preparation of single cell suspensions, and the expression of GFP was analyzed with flow cytometry 48 hours post infection. (B) Human 293, A549 and HEp‐2 cells were infected with fiber1‐modified viruses or FAdV4‐GFP at various MOIs for 4 hours. GFP expression was observed under a fluorescence microscope 48 hours post infection, and the results of F1CDR and F1IJR are demonstrated. (C) The percentages of GFP+ cells were determined by flow cytometry after microscopic observation. Chicken LMH served as the cell line control. All of the experiments were performed in duplicate and the data shown are from one representative experiment out of the three performed. ***p < 0.001