FIGURE 4.

Fiber2 knob replacement with that of HAdV‐35 in FAdV‐4 vector enhanced gene transduction to human suspension cells. Adherent or suspension cells were infected with FAdV4F1IJR‐EG (F1IJR‐EG) or FAdV4FIJ35K‐EG (FIJ35K‐EG). F1IJR‐EG carried RGD4C insertion in the IJ loop of the fiber1 knob and the human EF1a promoter‐controlled GFP expression cassette. FAdV‐4 fiber2 knob in F1IJR‐EG was replaced with that of HAdV‐35 to generate FIJ35K‐EG. HAdV5‐GFP was an E1/E3‐deleted HAdV‐5 carrying the CMV promoter‐controlled GFP expression cassette. GFP expression was observed under a fluorescence microscope (A) before determined by flow cytometry 48 hours post infection. The results of flow cytometry for adherent cells are shown in (B), and those for suspension cells are shown in (C) and (D). Fiber knob replacement slightly improved GFP expression in adherent 293 and HEp‐2 cells but not in LMH or A549 cells; and a such modification significantly improved gene transduction in human suspension cells such as Jurkat, K562 and U937. All of the experiments were performed in duplicate and the data shown are from one representative experiment of the three experiments performed. *p < 0.05, **p < 0.01 and ***p < 0.001