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. 2021 Jun 11;23(10):e3368. doi: 10.1002/jgm.3368

TABLE 1.

Summary information of PCR primers

Fragment	Primer name	Sequence	Template	Product (bp)	Annotation
SacI‐CDR	1805FAV4F1Mf	gtccataggc tattacatct acatggtg	pMD‐FAV4Fs	119	SacI
SacI‐CDR	1805FAV4F1CDRr	agaaacagtc tccgcggcag tcacagctcg cgcctgtgag gtc	pMD‐FAV4Fs	119
CDR‐AgeI	1805FAV4F1CDRf	tgactgccgc ggagactgtt tctgcggaga aaacagcctg actagcg	pMD‐FAV4Fs	475
CDR‐AgeI	1805FAV4F1Mr	accctgatag gaaaaaggga tagga	pMD‐FAV4Fs	475	AgeI
SacI‐DER	1805FAV4F1Mf	gtccataggc tattacatct acatggtg	pMD‐FAV4Fs	209	SacI
SacI‐DER	1805FAV4F1DERr	agaaacagtc tccgcggcag tcacagatga gggacaaatt cacctctgt	pMD‐FAV4Fs	209
DER‐AgeI	1805FAV4F1DERf	tgactgccgc ggagactgtt tctgcgtgcc gcccacggtc tc	pMD‐FAV4Fs	385
DER‐AgeI	1805FAV4F1Mr	accctgatag gaaaaaggga tagga	pMD‐FAV4Fs	385	AgeI
SacI‐HIR	1805FAV4F1Mf	gtccataggc tattacatct acatggtg	pMD‐FAV4Fs	443	SacI
SacI‐HIR	1805FAV4F1HIRr	agaaacagtc tccgcggcag tcacatccgc tggacgtgtt ctgg	pMD‐FAV4Fs	443
HIR‐AgeI	1805FAV4F1HIRf	tgactgccgc ggagactgtt tctgcaccac gccgtcggat gc	pMD‐FAV4Fs	151
HIR‐AgeI	1805FAV4F1Mr	accctgatag gaaaaaggga tagga	pMD‐FAV4Fs	151	AgeI
SacI‐IJR	1805FAV4F1Mf	gtccataggc tattacatct acatggtg	pMD‐FAV4Fs	521	SacI
SacI‐IJR	1805FAV4F1IJRr	agaaacagtc tccgcggcag tcacacgcgt tctggtcaaa ccagtt	pMD‐FAV4Fs	521
IJR‐AgeI	1805FAV4F1IJRf	tgactgccgc ggagactgtt tctgccccga cactgtggtg acgac	pMD‐FAV4Fs	73
IJR‐AgeI	1805FAV4F1Mr	accctgatag gaaaaaggga tagga	pMD‐FAV4Fs	73	AgeI
MauBI‐F2CDR	1811MD‐FAV4FSf	ccggtacctt tcggagggcg acg	pMD‐FAV4Fs	2245	MauBI
MauBI‐F2CDR	1811MD‐F2CDRp2	cagaaacagt ctccgcggca gtcacaccca gggcgattcc ccatgg		2245
F2CDR‐SbfI	1811MD‐F2CDRp3	gtgactgccg cggagactgt ttctgcgacc tcaactccgc caatgccaaa t	pMD‐FAV4Fs	787
F1‐F2CDR	1811MD‐FAV4FSr	gatatcctcc tttggaccca tggtagtcca c		3007	SbfI
F1IJR‐F2TS	1811MD‐FAV4FSf	ccggtacctt tcggagggcg acg	pKFAV4F1IJR‐GFP	2084	MauBI
F1IJR‐F2TS	1905F2TSF35Kp2	ggtgttaata ctatcgggtg tggagacgct cccc	pKFAV4F1IJR‐GFP	2084
F35K	1905F2TSF35Kp3	gcgtctccac acccgatagt attaacacct tatggactgg	pFiber5‐35	617
F35K	1905F2TSF35Kp4	gatcatggtt ccgctttagt tgtcgtcttc tgtaatgtaa g	pFiber5‐35	617
F2‐SbfI	1905F2TSF35Kp5	aagacgacaa ctaaagcgga accatgatcg tgg	pMD‐FAV4Fs	418
F1IJR‐F35K	1811MD‐FAV4FSr	gatatcctcc tttggaccca tggtagtcca c		3061	SbfI
Fiber‐seq	1805FAV4FMS1	taaccaatat cttctaggct ccg	plasmids or viruses with RGD4C insertion	1436	For identification
Fiber‐seq	1805FAV4FMS2	gaaaggataa accaggtcaa gc		or 1463	For identification
GFP‐frag	2008GFPf	gacaaccact acctgagcac cc	virus genomic DNA	126
	2008GFPr	cttgtacagc tcgtccatgc c	virus genomic DNA	126
	2008GFP‐probe	tccgccctga gcaaagaccc caac			5′HEX, 3′BHQ1