TABLE 2.
Summary information of purified FAdV‐4 vectors
| Virus name | Short name | Fiber modification | Deletion of FAdV‐4 genes | Promoter of transgene | Physical titer (× 1011 vp/ml) | Infectivity titer (× 109 IU/ml) | Particle‐to‐IU ratio |
|---|---|---|---|---|---|---|---|
| FAdV4‐GFP | None | ORF1, ORF1B, ORF2 | CMV promoter | 15 | 3.5 | 410 | |
| FAdV4F1CDR‐GFP | F1CDR | RGD4C in fiber1 CD loop | ORF1, ORF1B, ORF2 | CMV promoter | 12 | 2.5 | 480 |
| FAdV4F1DER‐GFP | F1DER | RGD4C in fiber1 DE loop | ORF1, ORF1B, ORF2 | CMV promoter | 22 | 1.1 | 1900 |
| FAdV4F1HIR‐GFP | F1HIR | RGD4C in fiber1 HI loop | ORF1, ORF1B, ORF2 | CMV promoter | 9.7 | 2.9 | 330 |
| FAdV4F1IJR‐GFP | F1IJR | RGD4C in fiber1 IR loop | ORF1, ORF1B, ORF2 | CMV promoter | 6.6 | 1.3 | 520 |
| FAdV4F2CDR‐GFP | F2CDR | RGD4C in fiber2 CD loop | ORF1, ORF1B, ORF2 | CMV promoter | 3.2 | 0.4 | 800 |
| FAdV4F1IJR‐EG | F1IJR‐EG | RGD4C in fiber1 IR loop | ORF0, ORF1, ORF1B, ORF2, ORF19A | human EF1a promoter | 15 | 2.5 | 620 |
| FAdV4FIJ35K‐EG | FIJ35K‐EG | RGD4C in fiber1 IR loop; replacement of fiber2 knob with that of HAdV‐35 | ORF0, ORF1, ORF1B, ORF2, ORF19A | human EF1a promoter | 20 | 1.1 | 1900 |
Vp, viral particle; IU, infectious unit.