Figure 3.

Investigation of roles of secondary metabolites in the soft rot disease. A) 10 μL of purified 9 and 8, a crude extract of 7, tolaasin I (all 500 μg mL⁻¹), and water were spotted onto mushroom slices and incubated for 48 hours at 30 °C. Brown spots indicate lesions. B) Exemplary photos of A. bisporus mycelium grown on agar plates with the indicated additives and C) area of mycelium grown; Tebu, tebuconazol; DMSO, dimethyl sulfoxide; PDB, potato dextrose broth extract. An asterisk marks significant results (Statistic standard student t‐test; p‐value <0.05; two‐tail). D) Extracted ion chromatogram of metabolic profiles of B. gladioli pv. agaricicola wild type (WT) and knockout strains; ▵spg, ▵cay, ▵tox, null producers of 8, 7 and 9, respectively. E) Infection assay of mushroom slices with B. gladioli pv. agaricicola wild type and indicated knockout strains as well as a caryoynencin‐KO complemented with 5 μL (2 μg μL⁻¹) of a crude extract enriched with 7.